Flag tag mouse monoclonal antibody
WebAntibody was analyzed by western blot using various amino-terminal and carboxy-terminal DDDDK fusion proteins and Invitrogen Positope" R900-40. A 1/5000 dilution gave bands against 0.1, 1.0 and 10 ng of the fusion proteins and 100 ng and 25 ng of the Positope. ELISA. Use a concentration of 1 - 5 µg/ml. As coating antibody in sELISA. WebThe DYKDDDDK tag, commonly referred to as Sigma®'s FLAG® Tag, is often used as a protein modification in order to simplify the labeling and detection of proteins.This unique amino acid sequence allows for specific antibody detection in western blotting, immunoprecipitation, and immunostaining techniques.Due to the short sequence, this …
Flag tag mouse monoclonal antibody
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WebAnti-DYKDDDDK (FLAG® epitope Tag) Antibody Features Suitable for Western Blot / WB application Broad-spectrum Recognition Capability Detect N and C terminal DYKDDDDK Tag in fusion proteins Anti-DYKDDDDK mouse monoclonal antibody at 1:1000 dilution. Sample: Recombinant Protein, 10ng/lane Lane A to K: Proteins with C-terminal FLAG® Tag WebFlag Tag Antibody is generated against 1xFlag tag (DYKDDDDK) and can recognize protein containing one or more Flag tags, independently on N-terminal, C-terminal or internal regions of the target protein. Target Information
WebThe antibody recognizes the DYKDDDDK peptide (the same epitope recognized by Sigma's Anti-FLAG® antibodies) fused to either the amino- or carboxy-terminus of targeted proteins. The binding specificity of this … WebAnti-FLAG Antibody Products The following lists a number of FLAG antibodies from various suppliers. The target antigen refers to the FLAG epitope tag, which is an octapeptide containing the amino acid sequence DYKDDDDK. Such antibodies can be used to detect or stain Flag-tagged recombinant proteins. Examples of FLAG antibody …
WebMouse monoclonal [9E10] to Myc tag Suitable for: ICC/IF, Flow Cyt, WB, IP, ELISA, IHC-Fr, Purification Reacts with: Recombinant fragment Isotype: IgG1 Related conjugates and formulations Alexa Fluor® 488 Alexa Fluor® 594 Alexa Fluor® 647 APC Biotin Biotin DyLight® 488 DyLight® 550 DyLight® 650 FITC HRP PE SureLight® APC See all (13)
WebMonoclonal Antibody for studying Flag-Tag. Cited in 855 publications. Validated for WB, IP, IHC, IF, F, ChIP. Highly specific and rigorously validated in-house, DYKDDDDK Tag (D6W5B) Rabbit Monoclonal … how many deadlifts should i dohttp://www.engibody.com/products/DYKDDDDK-FLAG-tag-Mouse-mAb-conjugated-Agarose-Beads-AT0078.html how many deadly sins are there in the bibleWebA FLAG tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. how many deadly sins in the bibleWebMany recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This antibody can detect either N-term or C-term HA tag. 产品编号. 产品名称. 包装. AG8057. HA Tag Rabbit Monoclonal Antibody. 50µl. AZ050. how many deadly animals live in australiaWebDYKDDDDK Tag (9A3) Mouse mAb detects exogenously expressed DYKDDDDK proteins in cells. The antibody recognizes the DYKDDDDK peptide, which is the same epitope recognized by Sigma's Anti-FLAG ® … how many deadly animals in australiaWebMouse monoclonal ANTI-FLAG ® M2 antibody was used as a counterstain at 1/500 dilution, and AlexaFluor ® 647 Goat anti-mouse secondary … how many deadly fungal infectionsWebDYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, independently on N … high tech high enrollment