Irf3 phosphorylation antibody
WebFigure Legend Snippet: TAOK1 promotes TBK1-IRF3 complex formation. a HEK293T cells were transfected with plasmids encoding HA-TBK1, Flag-IRF3, and varying doses of a plasmid encoding Myc-TAOK1 (0.5, 1.0, and 1.5 μg). Cells were harvested 24 h after transfection for immunoblot analysis as indicated with the antibody to Flag tag. b … WebDec 15, 2014 · To directly observe the distribution of HSPD1 and activated IRF3, an antibody specific to phosphorylated IRF3 (phospho S386) was used. Not surprisingly, overexpression of MAVS for 16 h resulted in phosphorylation of IRF3. Furthermore, almost all of the activated IRF3 co-localized with HSPD1 at that time point .
Irf3 phosphorylation antibody
Did you know?
WebHere we show that STING stimulates IRF3 phosphorylation by the kinase TBK1 in an in vitro reconstitution system. Using this system, we identified a carboxyl terminal region of STING that is both necessary and sufficient to activate TBK1 and stimulate IRF3 phosphorylation. WebViral infection results in phosphorylation of IRF3, leading to its translocation to the nucleus, where it activates promoters containing IRF3-binding …
WebMar 23, 2001 · We have investigated the mechanisms of phosphorylation of IRF7 and its close relative, IRF3, during activation of IFN gene expression following Newcastle disease virus (NDV) infection. By all parameters tested, IRF3 and IRF7 appeared to be activated by the same mechanism. WebKnockout Tested Rabbit recombinant monoclonal IRF3 antibody [EPR2418Y]. Validated in WB, IHC, ICC/IF, Flow Cyt (Intra) and tested in Mouse, Human. ... DsRNA generated during the course of an viral infection leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization ...
WebInvitrogen Anti-Phospho-IRF3 (Ser396) Polyclonal, Catalog # 720012. Tested in Western Blot (WB) and Immunocytochemistry (ICC/IF) applications. This antibody reacts with Human … Web(C,D) 4b(Δ2-38) inhibits the phosphorylation and nuclear translocation of IRF3. Experiments were carried out as in AB, except that 4b(Δ2-38) was accessed. Source publication
WebKey features and details Rabbit polyclonal to IRF3 Suitable for: WB Reacts with: Human Isotype: IgG You may also be interested in Western blot …
WebMar 4, 2024 · IRF3 is a transcriptional factor that mediates expression of a large array of genes after viral infection. We hypothesized that DUBs might regulate the activity or stability of IRF3 and thereby facilitate or inhibit cellular antiviral responses. daiwa warranty formWebAll mutants lacked phosphorylation of the STING-S365 residue, IRF3, and STAT1 upon stimulation, indicating defects in IRF3 activation and type I IFN signaling (Fig. 1A and SI … biotechnology society of tanzaniaWebTBK1-dependent phosphorylation of IRF3 leads to its nuclear translocation and subsequent activation of transcriptional programs that ultimately lead to ... for 2 h at RT or over-night at 4°C. Primary antibodies used include: anti-pDNA-PKcs Ser2056 (ab124918, Abcam), anti-pDNA-PKcs Ser2056 (ab18192, Abcam) for mouse cell lines, anti-DNA-PKcs ... daiwa whip sparesWebKey features and details Rabbit polyclonal to IRF3 (phospho S396) Suitable for: WB Reacts with: Human Isotype: IgG You may also be interested in Primary Anti-IRF3 (phospho S386) … daiwa waggler rods 13ftWebPhospho-IRF3 (Ser396) Antibody (MA5-14947) in WB Frontiers in microbiology 2024 - FIGURE 2 Effects of Set-Met on the expression of MAVS protein and the phosphorylation of IRF-3 induced by PDCoV. LLC-PK cells were incubated with PDCoV for 1 h, and 2, 4, 8, and 16 muM of Se-Met was added. biotechnology skin careWebUse Anti-phospho-IRF-3 (Ser396) Antibody (Rabbit Polyclonal Antibody) validated in WB to detect phospho-IRF-3 (Ser396) also known as Interferon regulatory factor 3. Anti-phospho … daiwa us ballistic mq ltWebAntibodies that detect IRF3 can be used in several scientific applications, including Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry and Peptide array. These antibodies target IRF3 in Human, Mouse, Rat, Non-human primate and Porcine samples. Our IRF3 monoclonal, polyclonal, recombinant monoclonal and recombinant ... biotechnology software engineer