Shrna depletion
Splet30. sep. 2014 · The proliferation effect of each shRNA in each cell line was assessed by transducing a population of 11M cells with one shRNA-virus per cell and determining the … Splet29. mar. 2024 · As shown in Figure 2H, the shRNA-mediated depletion of TASK-3 also suppresses the pH-dependent potassium current in MCF-10F cells. These data clearly show that TASK-3 is a functional channel in both cell lines, and that the pH-dependent portion of the macroscopic potassium current is carried through TASK-3 channels.
Shrna depletion
Did you know?
Splet18. maj 2015 · Herein we used shRNA depletion and a novel lactate dehydrogenase (LDHA) inhibitor, GNE-140, to probe the role of LDHA in tumor growth in vitro and in vivo. In MIA … SpletAfter the shRNA infection, the researcher would enrich M phase cells by FACS analysis and recover the shRNA from the enriched cells. By identifying which shRNA(s) are enriched in …
Splet01. apr. 2024 · Here, we show that the colorectal cancer cell line SK-CO-1 lacks detectable ATM protein expression and is sensitive to the PARP inhibitor olaparib. Similarly, HCT116 …
SpletUBE2D3 depletion changed the global proteome, with the levels of proteins from metabolic pathways, in particular retinol metabolism, being the most affected. However, the impact of UBE2D3 depletion on the ubiquitinome was much more prominent. Interestingly, molecular pathways related to mRNA translation were the most affected. Splet30. sep. 2014 · The proliferation effect of each shRNA in each cell line was assessed by transducing a population of 11M cells with one shRNA-virus per cell and determining the relative enrichment or depletion of ...
Splet04. jan. 2024 · This study establishes that conditional depletion of MCT4 profoundly impairs self-renewal and reduces the frequency and tumorigenicity of aggressive, therapy-resistant, glioblastoma-stem-cells...
SpletSETDB1 depletion and selective H3K9me3 loss in compartment A After terminal differentiation, mouse bone marrow-derived macrophages were infected with purified lentiviruses expressing two nontargeting (NT) shRNAs or two distinct shRNAs specific for SETDB1, causing a depletion of RNA and protein >90% ( Supplemental Fig. S1A ). ina garten black beans and riceSplet21. okt. 2011 · Previous screens have shown similar levels of shRNA representation to be suitable for the detection of shRNA depletion and enrichment [3, 4]. Since barcode … ina garten black eyed peas recipeSpletMA plots for two representative examples of simulated data from shRNA pooled screens with in silico twofold enrichment and depletion of shRNAs based on (A) Screen 100_2x … ina garten best roast chicken recipeSplet19. sep. 2024 · Using a comprehensive dataset of 501 cell lines screened with shRNA on a genome-scale level, the CTD 2 team found that shRNA depletion scores for pairs of … ina garten black and white cookie recipeSpletThe positive controls—shRNA or sgRNA that target well-known essential genes—displayed significant depletion in all four screens. We did note much higher variability in the shRNA … ina garten blackberry cobblerSplet27. maj 2024 · A virus expressing a short hairpin RNA (shRNA) targeting E2F1 and a TMOD2 overexpression virus were obtained from GenePharma Co., Ltd. (Shanghai, China) and used for infection. The E2F1 targeted shRNA sequence was as follows: ... To investigate the impact of E2F1 depletion on the CRPC cell lines, we assessed apoptosis, proliferation, … ina garten black eyed peasSpletThe expression of interleukin-12 and interferon-γ in the lymph nodes was enhanced by injection of TSP-1 shRNA. Lymphocytes from the mice injected with TSP-1 shRNA selectively killed the tumor cells, and the cytotoxicity of lymphocytes was abolished by depletion of CD8 + T cells. ina garten blonde brownie recipe